Old age chronic to breathe depleted the intersection of patient and blood platelet activate factor and blood platelet function change _'s symptom

Old age chronic to breathe depleted the intersection of patient and blood platelet activate factor and blood platelet function change _'s symptom
Old age chronic to breathe depleted the intersection of patient and blood platelet activate factor and blood platelet function change

Chinese geriatrics magazine Vol. 19 of number 3 of 2000 Clinical research

Author: Yu HuaPeng Liu LanPing Luo Mu's sum The king is prosperous and strong Liu YuHua

Unit: King Yu HuaPeng's prosperous and strong the Liu 's (510282 Guangzhou, the first division of respiratory disease of Pearl River hospital of army medical university) ; Liu LanPing ( Blood screening test lab) ; Luo Mu and ( Blood screening test lab)

Keyword: The breathing function is not complete; The blood platelet activates the factor; The blood platelet function is tested

[Summary ] Purpose Canvass old age to be chronic to breathe depleted the intersection of patient and blood platelet activate factor (PAF) And the blood platelet is adhered to, gathered, change of the release function and with chronic lung worry and relation of breathing depleted development of old age.Method Living beings quantitative law chronic to breathe the depleted group's patient to observe old age ' 55) , old age healthy people's group ' 40) And not the healthy people's group of old age ' 35) The blood platelet activates the factor (PAF) Change,it is if you can't last blood platelet membrane GMP140, blood platelet release function, blood platelet, last function (PADT) not to last law Gather the function (PAgT) with the blood platelet Change.Result Chronic to breathe depleted group old age PAF (171 51) Nmol/L is higher than the healthy people of old age (132 40) nmol/L( P

YU Huapeng

(Department of Respiratory Diseases, Zhujiang Hospital, The First Military Medical University. Guangzhou 510282,China)

LIU Lanping

(Department of Respiratory Diseases, Zhujiang Hospital, The First Military Medical University. Guangzhou 510282,China)

LUO Muhe, et al.

(Department of Respiratory Diseases, Zhujiang Hospital, The First Military Medical University. Guangzhou 510282,China)

[Abstract] ObjectivePlatelet activating factor (PAF) plays a key role in platelet activation.During the process of aging there will be dysfunction of endo thelium and thus inducing abnormal function of platelet and thrombosis. Our purpose is to investigate the change of PAF in chronic respiratory failure and its effect on plat elet function. MethodsPAF in plasma was detected and the function of platelet was determined by platelet adhesion test (PADT) ,platelet aggregation test (PAgT) and platelet grarule membrane (GM140) measurement in 55 elderly patients with chronic respiratory failure, 40 cases of healthy elderly subjects and 35 cases of healthy non-elderly subjects. ResultsPAF level in the elderly patients with chronic respiratory failure was significantly higher than that in healthy elderly subjects (PMaterial and method

First, observe the target

1.Old age chronic to breathe the depleted group ' Abbreviated as the old age and breathed out and declined the group) : 55, 36 men, 19 women, 65-81 years old, 67 average years olds. It is lung worry that amalgamates chronically and breathes the depleted patient, accord with " diagnostic standard that the second Meeting of national lung worry is made " (1977) . Among them, chronic bronchitis amalgamates obstructive 15 cases of pulmonary emphysema, 6 for 34 chronic lung worries, bronchiectasis.

2.Old health group: It is the elderly checking the body healthily, without clear heart and lungs disease, amounts to 40, 62-72 years old, 64 average years olds.

3.Non- old health group: 35, 20 men, 15 women, 35-58 years old, 44 average years olds.

Second, inspection method

1.The blood plasma PAF is determined: Consult Croft law ( 3)Draw in the blood the blood platelet of haemolysis activates the factor (lyso-PAF) . Then turn under anhydrous pyridine and function of vinegar anhydride into PAF. Extract add rabbit wash rich the intersection of blood platelet and thick liquid ' Blood platelet is several 500* 109 Mol/L, contains aspirin 5 mol/L, creatine phoshate 300 mg/L and creatine phosphokinase 150 mg/L) China. Determine the blood plasma draws the gathering rate that PAF washed the blood platelet to the rabbit on the blood platelet gathers the appearance, according to PAF standard product ' Products of U.S.A. Sigma Company) The quantity result that different density causes the blood platelet to gather reacts in the curve and converts the actual density in the thick liquid of bleeding.

2.The blood platelet function is determined: The blood platelet adheres to the function to determine that adopts the glass pearl law, the blood platelet gathers the function to determine and adopt than the turbid law, with two phosphoric acid nucleoside (ADP) In order to gather inducing pharmaceuticals, end the density is 6* 10-6mol/L. Blood platelet the intersection of release and function determine, adopt, set free, exempt law's finding the membrane albumen GMP140 (platelet granule granular membrane) of blood platelet ,It is tested that the kit is offered by Suzhou medical college.

Third, statistics are dealt with

All group data count to show by arithmetic,it is materials t examine after mating not to adopt.

Result

First, artery vim and vigour measure the result

Old age chronic to breathe out, decline the intersection of patient and the intersection of arterial blood and partial pressure of oxygen (PaO2)It is 9 (47 ) Mm Hg, the carbon dioxide of arterial blood (PaCO2)It is 11 (50 ) mm Hg; Old health group PaO2It is 10 (59 ) Mm Hg, PaCO2It is 8 (39 ) mm Hg; Non- old health group PaO2(72 13) Mm Hg, PaCO2(36 11) mm Hg.

Second, old age chronic to breathe out, decline the intersection of patient and blood platelet activate change of factor

Old age chronic to breathe out, decline patient blood plasma density of PAF ' 171 51) More old health group nmol/L (133 40) Nmol/L raises (P<0.01) notably ,Higher than non- old the intersection of health and group notably ' 115 32) nmol/L( P< 0.01) . Group's blood plasma PAF level of old health is higher than the non- old health group (P<0.05) .

Third, old age chronic to breathe out, decline patient's blood platelet adhere to the function and assemble the change of the function

Old age chronic to breathe out, decline the intersection of patient and blood platelet adhere to function (PADT) It is 18 (55 ) %, is higher than the control group (35 11) %(P< 0.01) ,Also old the intersection of health and group ' 44 13) % is obviously raised (P<0.01) . Old age chronic to breathe out, decline the intersection of patient and blood platelet gather function (PAgT) It is 18 (60 ) %, non- more old health group (37 8) % is obviously raised (P<0.01) ,Higher than old the intersection of health and group obviously ' 49 14) %(P< 0.01) .

Fourth, old age chronic to call and decline patient's blood platelet release function change

Old age chronic to breathe out, decline patient blood platelet membrane GMP140 ' 4 813 1 808) Molecule count blood platelet, old age relatively healthy (3 403 814) of people The molecule counts the blood platelet is raised (P<0.01) notably ,Also non- old the intersection of health and group ' 1 978 723) The molecule counts the blood platelet is obviously raised (P<0.05) .

Discussion

With the years, the aging phenomenon of the elderly's blood vessel wall is becoming more serious, stress that is shown as the endothelial cells of blood vessel degenerate and protect the function to drop, if is anticoagulatory and resisting the blood platelet function to weaken( 1,2). Chronic to breathe the depleted pathology that is lung source heart disease and change the foundation. Oxygen deficiency, acidosis can also cause the blood platelet function to be unusual and blood vessel endothelial cells are damaged besides causing arteriolar convulsion of the lung, promote the arteriolar little thrombosis of lung, cause artery high pressure of lung, right ventricular hypertrophy finally. The blood platelet function is unusual and releases the inflammation medium to play and act on greatly among them. The research previously reveals: Inflammation damage ADP, thrombese, collagen, 5 hydroxy color amine that release under the situation ' 5-HT) , Epinephrine, fibrin cracked product have very strong blood platelet to gather function. Compared with the above material, PAF is that the strongest blood platelet found so far gathers pharmaceuticals. It is low to 1* in the density 10-9Mol/L can cause rabbits to wash the blood platelet and assemble. Pour into 1* for guinea pig's mesenterium artery 10-6Mol/L PAF can cause thrombosis( 4,5). In addition, PAF also has extensive biological activity, such as inducing a cell and the monocyte to assemble and adhere to, increase trending towards the melting and releasing the inflammation medium, breathe out and decline a patient to hit PAF and blood platelet function and change more obviously of cells. Brief on at the intersection of blood platelet and the intersection of function and unusual foundation, and blood platelet function hyperfunction.

PAF is a kind of inflammation medium released by a cell, monocyte, macrophage, endothelial cell, blood platelet,etc., come from analog 1 of a kind of phosphatide acyl choline on the cell membrane - alkyl - 2 - acyl - sn - glycerine - phosphoric acid choline of lipoprotein. Through reducing cAMP level, promote phosphatide acyl inositol (phosphatidylinositol, PI) Decompose and Ca2+Indraft, cause the blood platelet to adhere to, assemble and release. Meanwhile, PAF still promotes the alkene sour supersession of the peanut four to produce the thrombus plain A2( TXA2), promote particle release 5 - HT, ADP,etc., strengthen vascular inflammation react with the blood platelet assembling further( 6,7,9). The membrane albumen GMP140 of blood platelet is a kind of specific albumen on the - particle membrane of blood platelet, leave over and become the specific mark on the membrane of blood platelet after the blood platelet releases the particle. In addition, GMP140 has function of adhering to the molecule, can lie between and lead blood platelet, neutral cells and monocyte to adhere to, aggravate inflammation and react ( 10). In a word, old age chronic to breathe depleted the intersection of patient and PAF and blood platelet function, worth further further investigating.

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(Accept draft date: 1999-08-27)
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